New

Research Laboratory Technician

Brigham and Women's Hospital
United States, Massachusetts, Brookline
850 Boylston Street (Show on map)
May 02, 2025
Primary Responsibilities: Anaerobic Microbiology: Perform bacterial culturing, including assays to assess bacterial growth and metabolic activity. Metabolite Extraction and Analysis: Extract metabolites from bacterial cultures and characterize using liquid chromatography-mass spectrometry. Bacterial Genetics: Perform bacterial genetic cloning and recombinant DNA techniques, including PCR, plasmid construction, site-directed mutagenesis, transposon library development and bacterial transformation. Clinical Sample Processing: Process patient samples (e.g., stool, biopsies) for multi-omics characterization, including DNA, RNA, Protein, and metabolite extractions. Collaboration and Training: This position is expected to train and work with a physician-scientist clinical research fellow, and additionally collaborate with an established postdoctoral research fellow in the lab. A successful candidate will be able to take direction, work independently, and collaborate to solve problems. Additional Responsibilities: Culture Preparation and Maintenance: Prepare and maintain anaerobic and aerobic bacterial cultures, including preparing specialized media for gut microbiome research. LC-MS Operation: Operate and maintain LC-MS systems. Data Management: Maintain accurate and detailed records of experimental procedures and results. Laboratory Maintenance: Ensure the team's workspace is clean, organized, and stocked with necessary supplies. Clinical Study Support (Optional): Assist with clinical studies, including sample collection and obtaining patient consent, if interested and time allows. Qualifications: Bachelor's or higher degree in biological sciences (microbiology, biochemistry, cell biology, or a related field). Demonstrated experience in microbiology, preferably including anaerobic culturing techniques. Preferred experience in bacterial genetics (PCR, site-directed mutagenesis, and recombinant DNA techniques) and/or metabolomics (mass spectrometry). Excellent organizational and time management skills. Strong communication and interpersonal skills. The successful candidate will be able to take direction, work independently, and collaborate with supervisors to solve problems. Physical Requirements Standing Frequently (34-66%) Walking Frequently (34-66%) Sitting Occasionally (3-33%) Lifting Frequently (34-66%) 35lbs+ (w/assisted device) Carrying Frequently (34-66%) 20lbs - 35lbs Pushing Occasionally (3-33%) Pulling Occasionally (3-33%) Climbing Rarely (Less than 2%) Balancing Frequently (34-66%) Stooping Occasionally (3-33%) Kneeling Occasionally (3-33%) Crouching Occasionally (3-33%) Crawling Rarely (Less than 2%) Reaching Frequently (34-66%) Gross Manipulation (Handling) Frequently (34-66%) Fine Manipulation (Fingering) Frequently (34-66%) Feeling Constantly (67-100%) Foot Use Rarely (Less than 2%) Vision - Far Constantly (67-100%) Vision - Near Constantly (67-100%) Talking Constantly (67-100%) Hearing Constantly (67-100%) The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.